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적혈구 생성 및 기형 억제작용

  • 관리자 (sinbio)
  • 2019-02-21 10:56:00
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적혈구 생성 및 항 최기 성 작용
1.골수에서  조혈 모세포의 증식을 돕는다.
2.엽산과 같이 항 최기 성 작용이 있다.
1. Zhongguo Zhong Xi Yi Jie He Za Zhi. 2004 Feb;24(2):127-9, 132
[Effect of panaxadiol saponin and panaxtriol saponin on proliferation of human bone marrow hemopoietic progenitor cells]
Niu YP, Qian XD, Wang WX.College of Pharmacy, Zhejiang University of Technology, Hangzhou 310014. niuyangping@hotmail.com
OBJECTIVE: To observe the effect of panaxadiol saponin (PDS) and panaxtriol saponin (PTS) on proliferation of human bone marrow hemopoietic progenitor cells (HPC). METHODS: PDS and PTS were separated and purified from ginsenosides, and the effects on HPC were studied using in vitro hemopoietic progenitor cell colony-forming technique, by observing the proliferation of human burst forming unit-erythroid progenitor (BFU-E), colony-forming unit-erythroid (CFU-E), colony-forming unit-granulocyte/macrophage (CFU-GM) and colony-forming unit-pluripotent hemopoietic progenitor (CFU-Mix) in mice after PDS and PTS stimulation. RESULTS: Different concentration of PDS (2.5-200 micrograms/ml) could stimulate the proliferation of HPC obviously, showing increase of CFU-E, BFU-E, CFU-GM and CFU-Mix by 54.9 +/- 6.3%, 48.8 +/- 5.1%, 27.6 +/- 4.2% and 48.9 +/- 3.9% respectively, which was higher than that of the control group. While stimulated by PTS of the same concentration, the CFU-E and BFU-E was lower than that of control significantly (P < 0.05); when the terminal concentration of PTS was 200 micrograms/ml, CFU-E and BFU-E was zero respectively. In the CFU-GM culture, PTS in concentration of 12.5 micrograms/ml could cause the proliferation increased by 29.7 +/- 2.2% (P < 0.05), but in concentration of 100 micrograms/ml and 200 micrograms/ml, it showed inhibitory effect on CFU-GM, the inhibition rate being 48.6 +/- 3.9% and 100% respectively. CONCLUSION: PDS is the effective component of ginsenosides in stimulating proliferation of human bone marrow HPC. PTS is an component with inhibitory action on proliferation of CFU-E and BFU-E and its effect on CFU-GM was depending on its concentration.
인간 골수 조혈모세포(hemopoietic progenitor cells:HPC)의 증식에 미치는 PPD사포닌(PDS)와 PPT계 사포닌의(PTS) 의 영향
방법:진세노사이드로부터 PDS와 PTS를 순수 분리하여 실험관내 실험에서 HPC 콜로니 생성 기술을 이용하여 연구하였다(인간 격발 형성 단위 적혈모세포(BFU-E)의 증식, 콜로니 형성 단위 적혈(CFU-E), 콜로니 형성 단위 과립구/대식세포(CFU-GM)과 콜로니 형성 단위 pluripotent 적혈모세포(CFU-Mix)를 pds와 pts로 생쥐를 자극 한 후 관찰)
결과: PDS의 2.5-200 micrograms/ml는 HPS 의 증식을 명백히 자극하였다(CFU-E:  54.9 +/- 6.3%, BFU-E%: 48.8 +/- 5.1%, CFU-GM: 27.6 +/- 4.2%, CFU-Mix: and 48.9 +/- 3.9%로 비교적 대조 군에 비해 높았다) 반면에 PTS의 동일 농도로 자극 시 CFU-E and BFU-E 은 대조군에 비해 낮았으며200 micrograms/ml 농도에서는 CFU-E and BFU-E는 제로였다. CFU-GM 배양에서는 PTS농도 12.5 micrograms/ml은 by 29.7 +/- 2.2% (P < 0.05)만큼 증식을 증가시켰으나 100 micrograms/ml과 200 micrograms/ml 에서는 증가가 없었고 . 48.6 +/- 3.9% and 100% 의 억제 효과를 보였다. 
결론: PDS는 인간 골수에서 조혈 모세포의 증식을 자극하는 효과적 물질인 반면 PTS는 CFU-E and BFU-E의 증식에 억제 작용을 보이는 물질이며 CFU-GM에 대한 효과는 농도에 의존한다.
2.Zhongguo Zhong Xi Yi Jie He Za Zhi. 2000 Sep;20(9):673-6.
[Effect of ginsenosides on proliferation and differentiation of human CD34+ hematopoietic stem/progenitor cells]J
in J, Tao H, Gao R.Affiliated Hospital of Zhejiang College of TCM, Hangzhou (310006)
.OBJECTIVE: To investigate the effect of ginsenosides (GS) on proliferation and differentiation of human CD34+ stem/progenitor cells. METHODS: CD34+ hematopoietic progenitor cells were isolated from umbilical cord blood by using the immune beads sorting system. The cells were exposed to GS of different concentrations in both liquid culture and semi-solid culture, and the elevation rate on proliferation of CD34+ stem/progenitor cells and colony formation were estimated. The cells were marked with monoclonal antibody and the marker was examined by flow cytometry after incubated with GS for 14 days. RESULTS: GS (5-50 micrograms/ml) could raise the colony production rate of BFU-E, CFU-E, CFU-GM, CFU-GEMM by (87.6 +/- 2.6)%, (63.3 +/- 2.8)%, (58.0 +/- 3.1)% and (96.3 +/- 5.5)% respectively (all P < 0.01), and the best effect in improving cell proliferation of CD34+ cells in vitro was obtained when the concentration of GS was 25 micrograms/ml. After incubation with GS for 14 days, number of CD33+ cells was increased by GS in a dose-dependent manner with a peak increasing rate at 200 micrograms/ml. In the presence of GS 50 micrograms/ml, CD15+ cells were reaching the peak. Number of CD71+and G-A+ cells increased only when the concentration of GS was 25 micrograms/ml. CONCLUSION: GS could not only promote the proliferation but also induce the differentiation of CD34+ hematopoietic stem/progenitor cells, GS may play the role by cooperating with hematopoietic growth factor, and by its growth factor-like function in the regulation of hematopoiesis.PMID: 11789172 [PubMed - indexed for MEDLINE] 
MID: 15015445 [PubMed - indexed for MEDLINE
3. Fitoterapia. 2004 Jun;75(3-4):337-41.
Hematopoietic effect of ginsenoside Rg3 in ICR mouse primary cultures and its application to a biological response modifier.
Joo SS, Won TJ, Kim MS, Lee DI.Department of Immunology, College of Pharmacy, Chung-Ang University, Seoul 156-756, South Korea.
Ginsenoside Rg3,which is obtained as a by-product during the steaming of red ginseng, at 300 microg/ml enhanced the proliferation of the total spleen and bone marrow (BM) cells in both the cyclophosphamide (CYC)-treated and non-CYC-treated groups. 
Copyright 2004 Elsevier B.V.PMID: 15158992
 [PubMed - indexed for MEDLINE]
항 최기성 작용
5. Chin Med J (Engl). 2003 Nov;116(11):1776-8.
Protective effects of vitamin B12, ginseng saponin, and folic acid against murine(쥣과) fetal deformities caused by hyperthermia.Li H, Wang B, Zhao D, Han L.Department of Gynecology and Obstetrics, Second Hospital of Jilin University, Changchun 130021, China.OBJECTIVE: To investigate the protective effects of vitamin B(12), ginseng saponin, and folic acid on mouse embryos subjected to high heat. METHODS: Mice were used for the experiment. RESULTS: After exposure of pregnant mice to high heat, the rates of teratism, stillbirth, and fetal absorption were markedly lower in mice treated with ginseng saponin and folic acid following heat exposure than in untreated mice. There were no significant differences in these rates when comparing mice treated with vitamin B(12) with the untreated mice. CONCLUSIONS: Ginseng saponin and folic acid can lessen injuries to murine embryos caused by high heat, while vitamin B(12) has little protective effect against high temperature except for promoting overall embryonic growth.
쥣과 동물에서 고열로 유발된 태아 기형에 대한 VB12,진세노사이드, 엽산의 방어 효과
목적: 생쥐 태아를 고열에 노출 시켜 VB12와 진세노사이드, 엽산의 보호효과를 연구
방법: 생쥐를 실험용으로 사용함
결과: 임신한 생쥐를 고열에 노출 시킨 후 인삼 사포닌을 먹인 쥐에서 대조군에 비해  기형 및 사산 태아 흡수율이 현저히 낮았고 엽산이 다음이었고 VB12를 먹인 쥐에선 의미 있는 변화는 없었다.
결론: 인삼사포닌과 엽산은 쥣과 동물 태아에 고열로 야기된 손상을 감소시킬 수 있는 반면 VB12는 전반적인 태아의 성장을 증진하는 것 외에 효과는 없었다.
PMID: 14642159 [PubMed - indexed for MEDLINE] 
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